Detection of PD-1 for flow cytometry immunophenotyping using commercial antibodies in clinical studies with check point inhibitors
نویسندگان
چکیده
Abstract Precision for Medicine (Precision) has optimized the use of commercial anti-PD-1 and anti-IgG4 reagents to detect both free PD-1 and/or Pembro-bound in pharmacodynamic studies when Pembrolizumab, a concomitant check point inhibitor, is used combination with new therapy. developed qualified flow immunophenotyping assays quantify on CD4+ CD8+ T cells using clone (PD1.3.1.3) an anti-IgG4. This enabled detection drug bound receptor. We compared PHA stimulated healthy donors treated saturating Pembrolizumab or no treatment. There was not significant difference observed quantification presence absence which demonstrated that our approach able accurately measure total whether bound. As titrated from concentration there dose-dependent decrease PD-1. Here we will demonstrate above methodology other antibody clones. In clinical trials incorporate checkpoint inhibitors like either PD-L1, selection are key considerations these receptors designing sensitive, selective, accurate biomarker assay.
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ژورنال
عنوان ژورنال: Journal of Immunology
سال: 2023
ISSN: ['1550-6606', '0022-1767']
DOI: https://doi.org/10.4049/jimmunol.210.supp.246.02